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HEPES, 100.0% (NaOH titration), BioPerformance Certified®
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Research Use only: Not intended for animal or
human diagnostic or therapeutic use.
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Beilstein Number
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883043
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IUPAC name
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2-[4-(2-Hydroxyethyl)piperazin-1-yl]ethanesulfonic
acid
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Boiling point
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210-215°C
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MDL Number
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MFCD00006158
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CAS Number
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7365-45-9
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Melting point
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234°C
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Density
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1.325
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Lin formula
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C8H18N2O4S
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EINES Number
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230-907-9
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Mol Formula
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Flash point
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°C
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Molecular weight
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238.30
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Refractive index
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1.339
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Solubility
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in water
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Synonym(s)
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1-[4-(2-Hydroxyethyl)-1-piperazinyl]ethane-2-sulfonic
acid; 2-[4-(2-Hydroxyethyl)piperazin-1-yl]ethanesulfonic acid;
2-[4-(2-Hydroxyethyl)piperazinyl]-ethanesulfonic acid; 230-907-9;
4-(2-Hydroxyethyl)piperazine-1-(2-ethanesulfonic acid); 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic
acid; 7365-45-9; 883043; C8H18N2O4S; HEPES; MFCD00006158;
N'-(2-Hydroxyethyl)piperazine-N-(2-ethanesulfonic acid);
N-(2-Hydroxyethyl)piperazine-N'-(2-ethanesulfonic acid);
N-(2-Hydroxyethyl)piperazine-N'-2-ethanesulfonic acid; N-(2-Hydroxyethyl)piperazine-N'-ethanesulfonic
acid; N-[2-Hydroxyethyl]piperazine-N'-[2-ethanesulfonic acid];
N'-2-Hydroxyethylpiperazine-N-2-ethanesulfonic acid;
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Actual
Certificate of Analysis
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Part No. (SKU) 2309079-1BC
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Lot No. (MPN) 0914220219CL
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HTS Code (MPN) 2933.59.9500
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Appearance white
crystalline powder; Assay (NaOH titration) 100.0%; Endotoxin assay <0.05
eu/ml, <0.005 eu/mg; Heavy metals (as Pb) NMT 5ppm passes; Identification (FTIR
spectra) consistent with structure; Iron (Fe) NMT 5ppm passes; Rnase, Dnase,
Nickase and Protease none detected; Solubility (water) at 500mg/ml; Standard
plate count 0 cfu/mg; Suitability for cell culture; UV absorbance (33% w/w)
NMT 0.02 at 290nm, (0.1m) NMT 0.01 at 260nm, (0.1) NMT 0.007 at 280nm; Water
(H2O, KF) 0.05%;
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Applications
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Does not confer cytotoxic effects on cells and thus
can be used in animal cell cultures. Has been described as one of the best
all-purpose buffers available for biological research. At biological pH, the
molecule is zwitterionic, and is effective as a buffer at pH 6.8 to 8.2. Has
been used in a wide variety of applications, including tissue culture. It is
commonly used to buffer cell culture media in air. Finds usage in in vitro
experiments on Mg. Used to supplement Dulbecco's modified Eagle's medium to
culture and maintain cell lines. As a component of platelet suspension buffer.
To supplement Hank's basic salt solution, which is used to wash pancreatic
tissue. As a component of wash buffer and blocking buffer in the purification
and quantification of protein with enzyme-linked immunosorbent (ELISA) assay.
For the adjustment and maintenance of pH of biological solutions. As a
component of Hank's balanced salt solution (HBSS) and dissociation medium to
study neuronal development. For homogenization of tissue and in the
preparation of cytosolic and nuclear extract from cells. As a component of
keratinocyte and fibroblast culture medium.
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GHS (Accordance with 29 CFR 1910, OSHA HCS)
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Pictogram
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None
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Hazard statement(s)
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Safety
first! Do not handle until all safety precautions have been read (SDS) and
understood.
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NFPA RATING (National Fire Protection
Association)
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Health
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0
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Flammability
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0
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Instability
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0
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Special hazard
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Storage
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General
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TRANSPORTION INFORMATION
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US DOT
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Not dangerous goods
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Exemptions
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Not applicable
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