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HEPES, 100.4% (NaOH titration), Cell Culture Certified®
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Research Use only: Not intended for animal or
human diagnostic or therapeutic use.
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Beilstein Number
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883043
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IUPAC name
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2-[4-(2-Hydroxyethyl)piperazin-1-yl]ethanesulfonic
acid
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Boiling point
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210-215°C
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MDL Number
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MFCD00006158
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CAS Number
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7365-45-9
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Melting point
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234°C
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Density
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1.325
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Lin formula
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C8H18N2O4S
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EINES Number
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230-907-9
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Mol Formula
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Flash point
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°C
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Molecular weight
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238.30
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Refractive index
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1.339
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Solubility
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in water
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Synonym(s)
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1-[4-(2-Hydroxyethyl)-1-piperazinyl]ethane-2-sulfonic
acid; 2-[4-(2-Hydroxyethyl)piperazin-1-yl]ethanesulfonic acid;
2-[4-(2-Hydroxyethyl)piperazinyl]-ethanesulfonic acid; 230-907-9;
4-(2-Hydroxyethyl)piperazine-1-(2-ethanesulfonic acid); 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic
acid; 7365-45-9; 883043; C8H18N2O4S; HEPES; MFCD00006158;
N'-(2-Hydroxyethyl)piperazine-N-(2-ethanesulfonic acid);
N-(2-Hydroxyethyl)piperazine-N'-(2-ethanesulfonic acid);
N-(2-Hydroxyethyl)piperazine-N'-2-ethanesulfonic acid; N-(2-Hydroxyethyl)piperazine-N'-ethanesulfonic
acid; N-[2-Hydroxyethyl]piperazine-N'-[2-ethanesulfonic acid];
N'-2-Hydroxyethylpiperazine-N-2-ethanesulfonic acid;
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Actual
Certificate of Analysis
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Part No. (SKU) 2309079-1CCC
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Lot No. (MPN) 0914221253CL
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HTS Code (MPN) 2933.59.9500
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Appearance white
crystalline powder; Assay (NaOH titration) 100.4%; Heavy metals (Pb) 5ppm; Identification
(IR spectrum) consistent with structure; Suitability (mammalian) cell culture;
UV absorbance (at 290 nm, 33% w/w) 0.008; Water (H2O, KF) 0.02%
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Applications
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Been described as one of the best all-purpose buffers
available for biological research. At biological pH, the molecule is
zwitterionic, and is effective as a buffer at pH 6.8 to 8.2. HEPES has been
used in a wide variety of applications, including tissue culture. It is
commonly used to buffer cell culture media in air. HEPES finds its usage in
in vitro experiments on Mg. Used as a component in Danieau medium, which is
used to prevent melanin pigment formation post gastrulation of zebra-fish
embryo. As a component of Hanks' solution used in the degradation testing of
Iron. As a component of HEPES medium in the transfection of cell culture with
plasmids. Along with RPMI-1640, L-glutamine, FBS, Sodium pyruvate and
ß-mercaptoethanol for the culturing of rat insulinoma cells. As a supplement
in TCM199 medium for washing fresh oocytes required for nuclear transfer
studies
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GHS (Accordance with 29 CFR 1910, OSHA HCS)
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Pictogram
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None
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Hazard statement(s)
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Safety
first! Do not handle until all safety precautions have been read (SDS) and
understood.
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NFPA RATING (National Fire Protection
Association)
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Health
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0
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Flammability
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0
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Instability
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0
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Special hazard
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Storage
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General
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TRANSPORTION INFORMATION
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US DOT
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Not dangerous goods
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Exemptions
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Not applicable
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