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HEPES, 100.6% (C8H18N2O4S, free acid), Ultrapure BioReagent Certified®
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Research Use only: Not intended for animal or
human diagnostic or therapeutic use.
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Beilstein Number
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883043
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IUPAC name
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2-[4-(2-Hydroxyethyl)piperazin-1-yl]ethanesulfonic
acid
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Boiling point
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210-215°C
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MDL Number
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MFCD00006158
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CAS Number
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7365-45-9
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Melting point
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234°C
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Density
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1.325
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Lin formula
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C8H18N2O4S
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EINES Number
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230-907-9
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Mol Formula
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Flash point
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°C
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Molecular weight
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238.30
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Refractive index
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1.339
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Solubility
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in water
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Synonym(s)
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1-[4-(2-Hydroxyethyl)-1-piperazinyl]ethane-2-sulfonic
acid; 2-[4-(2-Hydroxyethyl)piperazin-1-yl]ethanesulfonic acid;
2-[4-(2-Hydroxyethyl)piperazinyl]-ethanesulfonic acid; 230-907-9;
4-(2-Hydroxyethyl)piperazine-1-(2-ethanesulfonic acid); 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic
acid; 7365-45-9; 883043; C8H18N2O4S; HEPES; MFCD00006158;
N'-(2-Hydroxyethyl)piperazine-N-(2-ethanesulfonic acid);
N-(2-Hydroxyethyl)piperazine-N'-(2-ethanesulfonic acid);
N-(2-Hydroxyethyl)piperazine-N'-2-ethanesulfonic acid; N-(2-Hydroxyethyl)piperazine-N'-ethanesulfonic
acid; N-[2-Hydroxyethyl]piperazine-N'-[2-ethanesulfonic acid];
N'-2-Hydroxyethylpiperazine-N-2-ethanesulfonic acid;
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Actual
Certificate of Analysis
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Part No. (SKU) 2309079-1UBC
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Lot No. (MPN) 0914221305CL
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HTS Code (MPN) 2933.59.9500
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Appearance white
crystalline powder; Assay (C8H18N2O4S, free acid) 100.6%; Arsenic and
Antimony (as As) <0.05%; Heavy metals (as Pb) <1ppm; Identification (IR)
conforms; Iron (Fe) <3ppm; pH (5% solution at 25°C) 5.31; pKa (at 20°C)
7.56; Rnase, Dnase, Nickase and Protease none detected; Residue after
ignition <0.02%; Sodium (Na, USP Id) passes; Solubility in water passes; Suitability
for (mammalian) cell culture; UV absorbance of a 0.1M Solution (1-cm path vs
water) 0.003 at 260nm, (1-cm path vs water) 0.002 at 280nm
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Applications
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For Liquid Chromatography and Molecular Biology applications.
High purity buffer for biological sciences, particularly used in cell culture
to maintain physiological pH. It acts a buffering component, which is used in
the preparation of buffers. It is described as one of the best all-purpose
buffers available for use in biological research. been described as one of
the best all-purpose buffers available for biological research. At biological
pH, the molecule is zwitterionic, and is effective as a buffer at pH 6.8 to
8.2. Used in a wide variety of
applications, including tissue culture. It is commonly used to buffer cell
culture media in air. Usage in in vitro experiments on Mg. Used as a
component of DMEM (Dulbecco's modified Eagle's medium) used for the
cultivation of CHO-GPR40 cells. RNA buffer (for gel-filtration
chromatography) and transcription buffer (for transcription reaction). Recapture
solution to re-capture RNA exosomes from the glycerol gradient. ExoI and
ExoII extraction solution as well as light and heavy gradient solution for
gradient centrifugation. 2X HBS buffer that is used in transfection process
for the production of recombinant lentivectors.
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GHS (Accordance with 29 CFR 1910, OSHA HCS)
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Pictogram
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None
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Hazard statement(s)
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Safety
first! Do not handle until all safety precautions have been read (SDS) and
understood.
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NFPA RATING (National Fire Protection
Association)
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Health
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0
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Flammability
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0
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Instability
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0
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Special hazard
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Storage
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General
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TRANSPORTION INFORMATION
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US DOT
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Not dangerous goods
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Exemptions
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Not applicable
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