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HEPES sodium salt, 99.95% (H2SO4 titration, anhydrous) Cell
Culture Certified®
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Research Use only: Not intended for animal or
human diagnostic or therapeutic use.
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Beilstein Number
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IUPAC name
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Sodium 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonate
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Boiling point
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°C
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MDL Number
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MFCD00036463
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CAS Number
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75277-39-3
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Melting point
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234°C
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Density
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Lin formula
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C8H17N2NaO4S
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EINES Number
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278-169-7
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Mol Formula
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Flash point
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°C
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Molecular weight
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260.29
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Refractive index
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Solubility
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in water
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Synonym(s)
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278-169-7;
4-(2-Hydroxyethyl)-1-piperazineethanesulfonic acid sodium salt; 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic
acid hemisodium salt; 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid
sodium salt; 75277-39-3; C8H17N2NaO4S; HEPES hemisodium salt; HEPES sodium
salt; MFCD00036463; N-(2-Hydroxyethyl)piperazine-N'-(2-ethanesulfonic
acid) sodium salt; Sodium
4-(2-hydroxyethyl)piperazin-1-ylethanesulphonate;
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Actual
Certificate of Analysis
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Part No. (SKU) 2781697-3N5CCC
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Lot No. (MPN) 0914221433CL
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HTS Code (MPN) 2933.59.9500
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Appearance spray dried crystallized
powder; Assay (H2SO4 titration, anhydrous) 99.95%; Endotoxin assay <0.01
eu/mg; Heavy metals (as Pb) 5ppm; ICP/MS analysis (Ag, As, Bi, Cd, Cu, Hg,
Mo, Pb, Sb, Sn) <5ppm; Identification (IR spectra) conforms to structure; Iron
(Fe) 5ppm; pH (1% aqueous solution) 10.08; Rnase, Dnase, Nickase and Protease
none detected; Solubility (water) at 1M at 20°C; Suitability for cell culture;
Total aerobic microbial count <10 cfu/g; UV absorbance (33% w/w) 0.05 at
290nm, (1M) 0.01 at 260nm, (0.1) <0.01 at 280nm; Water (H2O, KF) 0.04%;
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Applications
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Is a dipolar ionic buffer, useful for cell culture in
vitro, enzyme assays, and some electrophoretic applications at physiological
pH and for biological research. Its applications include, tissue culture,
Phosphorylation and photophosphorylation, fixative Transmission electron
microscopy, protein synthesis, preventing binding to non-receptor materials.
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GHS (Accordance with 29 CFR 1910, OSHA HCS)
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Pictogram
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None
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Hazard statement(s)
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Safety
first! Do not handle until all safety precautions have been read (SDS) and
understood.
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NFPA RATING (National Fire Protection
Association)
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Health
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0
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Flammability
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0
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Instability
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0
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Special hazard
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Storage
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General
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TRANSPORTION INFORMATION
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US DOT
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Not dangerous goods
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Exemptions
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Not applicable
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